in vitro anti-proliferative effect of adiponectin on human endometriotic stromal cells through adipor1 and adipor2 gene receptor expression
نویسندگان
چکیده
background: endometriosis is a complex disorder in reproductive age women which consist of stromal and epithelial cells implantation outside the uterine cavity. adiponectin is a member of cytokine family with various metabolic roles and proliferation inhibition of many cancer cells. the aim of the present research was to determine adiponectin effect on human endometriotic stromal cells (escs) proliferation and their expression of adiponectin receptors. methods: in this experimental study, endometrial biopsies (n = 7) were taken. escs isolation was done by enzymatic digestion and cell filtrations. escs of each biopsy were divided into four groups: 0 (control), 10, 100, and 200 ng/ml adiponectin concentrations in three different times (24, 48 or 72 h). the effect of adiponectin on esc viability and expression of mrna adipo receptor1 (r1) and adipo receptor2 (r2) was determined by trypan blue staining and semi-quantitative rt-pcr, respectively. data were analyzed by one-way anova and unpaired student’s t-test, and p < 0.05 was considered statistically significant. results: adiponectin inhibited human endometriotic stromal cell proliferation in time- and dose-dependent manners significantly (p = 0.001). expression of adipor1 and adipor2 gene receptors was increased in human escs significantly (p < 0.05). conclusions: adiponectin can suppress endometriosis by inhibiting esc proliferation and increased adipor1 and adipor2 expression.
منابع مشابه
in vitro Anti-Proliferative Effect of Adiponectin on Human Endometriotic Stromal Cells through AdipoR1 and AdipoR2 Gene Receptor Expression
Background: Endometriosis is a complex disorder in reproductive age women which consist of stromal and epithelial cells implantation outside the uterine cavity. Adiponectin is a member of cytokine family with various metabolic roles and proliferation inhibition of many cancer cells. The aim of the present research was to determine adiponectin effect on human endometriotic stromal cells (ESCs) p...
متن کاملin vitro Anti-Proliferative Effect of Adiponectin on Human Endometriotic Stromal Cells through AdipoR1 and AdipoR2 Gene Receptor Expression.
BACKGROUND Endometriosis is a complex disorder in reproductive age women which consist of stromal and epithelial cells implantation outside the uterine cavity. Adiponectin is a member of cytokine family with various metabolic roles and proliferation inhibition of many cancer cells. The aim of the present research was to determine adiponectin effect on human endometriotic stromal cells (ESCs) pr...
متن کاملAnti-Proliferative Effect of Adiponectin on Human Endometriotic Stromal Cells through AdipoR1 and AdipoR2 Gene Receptor Expression
Background: Endometriosis is a complex disorder in reproductive age women which consist of stromal and epithelial cells implantation outside the uterine cavity. Adiponectin is a member of cytokine family with various metabolic roles and proliferation inhibition of many cancer cells. The aim of the present research was to determine adiponectin effect on human endometriotic stromal cells (ESCs) p...
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متن کامل
adiponectin effect on the viability of human endometrial stromal cells and mrna expression of adiponectin receptors
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متن کاملExpression of adiponectin receptors, AdipoR1 and AdipoR2, in normal colon epithelium and colon cancer tissue.
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عنوان ژورنال:
iranian biomedical journalجلد ۲۰، شماره ۱، صفحات ۱۲-۱۷
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